Part:BBa_I718001:Design

ech My new test generator part Feruloyl CoA hydratase for vanilin

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 892
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 892
  Illegal NotI site found at 836
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 892
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 892
  Illegal NgoMIV site found at 265
  Illegal NgoMIV site found at 283
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Needs to be used in conjunction with fcs in order to generate vanilin.

It uses an ITPG promoter and an high efficiency RBS terminator.

Source

Uses No part name specified with partinfo tag. as well as a promoter, RBS, and terminator.